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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
29/05/2014 |
Data da última atualização: |
23/05/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ARGÔLO FILHO, R. C.; COSTA, R. L.; PINHEIRO, H. E.; CORRÊA, F. M.; VALICENTE, F. H.; POMELLA, A. W. V.; LOGUERCIO, L. L. |
Afiliação: |
FERNANDO HERCOS VALICENTE, CNPMS. |
Título: |
Requirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Toxins, v. 6, p. 1598-1614, 2014. |
DOI: |
10.3390/toxins6051598 |
Idioma: |
Inglês |
Conteúdo: |
Bioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activities and avoid the potential lack of identification of undesirable toxins. These findings are discussed in the contexts of commercial Bt product development, advantages of multiple-activity strains, and care and handling recommended for large-scale fermentation systems. MenosBioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activ... Mostrar Tudo |
Thesagro: |
Toxina. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02484naa a2200217 a 4500 001 1987282 005 2017-05-23 008 2014 bl uuuu u00u1 u #d 024 7 $a10.3390/toxins6051598$2DOI 100 1 $aARGÔLO FILHO, R. C. 245 $aRequirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development.$h[electronic resource] 260 $c2014 520 $aBioinsecticides with lower concentrations of endospores/crystals and without loss of efficiency are economically advantageo us for pest biocontrol. In addition to Cry proteins, other Bacillus thuringiensis (Bt) toxins in culture supernatants (SN) have biocontrol potential (e.g., Vip3A, Cry1I, Sip1), whereas others are unwanted (?-exotoxins), as they display widespread toxicity across taxa. A strain simultaneously providing distinct toxin activities in crystals and SN would be desirable for bioinsecticides development; however, strains secreting ?-exotoxins should be discarded, independently of other useful entomotoxins. Entomotoxicity of crystals and SN from a Brazilian Bt tolworthi strain (Btt01) was tested against Spodoptera frugiperd to assess the potential for biocontrol-product development based on more than one type of toxin/activity. Tests showed that 107 endospores mL?1 caused >80% of larvae mortality, suggesting Btt01 may be used in similar concentrations as those of other Bt-based biopesticides. When it was applied to cornfields, a significant 60% reduction of larvae infestation was observed. However, bioassays with Btt01 SN revealed a thermostable toxic activity. Physicochemical characterization strongly suggests the presence of unwanted ?-exotoxins, with isolate specific temporal variation in its secretion. Knowledge of the temporal pattern of secretion/activity in culture for all forms of toxins produced by a single strain is required to both detect useful activities and avoid the potential lack of identification of undesirable toxins. These findings are discussed in the contexts of commercial Bt product development, advantages of multiple-activity strains, and care and handling recommended for large-scale fermentation systems. 650 $aToxina 700 1 $aCOSTA, R. L. 700 1 $aPINHEIRO, H. E. 700 1 $aCORRÊA, F. M. 700 1 $aVALICENTE, F. H. 700 1 $aPOMELLA, A. W. V. 700 1 $aLOGUERCIO, L. L. 773 $tToxins$gv. 6, p. 1598-1614, 2014.
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Registros recuperados : 18 | |
7. | | ROCHA, M. C.; DELIZA, R.; CORRÊA, F. M.; ABBOUD, A. C. S.; CARMO, M. G. F. do. Aceitação de tomate cereja sob manejo orgânico de produção. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 21.; SEMINÁRIO LATINO AMERICANO E DO CARIBE DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 15., 2008, Belo Horizonte. Anais... Belo Horizonte: SBCTA, 2008. 1 CD-ROM.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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8. | | FREITAS, D. G. C.; SOUZA, R. C.; LATTARIO, F.; DIAS, M. S. P. C.; CORREA, F. M.; REIS, M. Alimentos industrializados: investigando a preferência do consumidor dentre alguns produtos do mercado. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 23., 2012, Campinas. Anais... Campinas: SBCTA, 2012. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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9. | | FREITAS, D. de G. C.; CORRÊA, F. M.; SILVA, J. H. M. da; DELIZA, R.; SOARES, A. G. Consumer perception of minimally processed pupunha heart palm. In: WORLD CONGRESS OF FOOD SCIENCE & TECHNOLOGY, 14., 2008, Shanghai, China. Abstracts. Beijing: Chinese Institute of Food Science and Technology, 2008. p. 440. TS20-17.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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11. | | ROCHA, M. C.; DELIZA, R.; CORRÊA, F. M.; ABBOUD, A. C. S; CARMO, M. G. F. do. Perfil sensorial de tomate do tipo cereja in natura. In: CONGRESSO BRASILEIRO DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 21.; SEMINÁRIO LATINO AMERICANO E DO CARIBE DE CIÊNCIA E TECNOLOGIA DE ALIMENTOS, 15., 2008, Belo Horizonte. Anais... Belo Horizonte: SBCTA, 2008. 1 CD-ROM.Tipo: Artigo em Anais de Congresso / Nota Técnica |
Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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13. | | FREITAS, D. G. C.; CORREA, F. M.; DELIZA, R.; SOUZA, A. L.; ALCANTARA, M.; GODINHO, J. C. C.; PEREIRA, P. C. A. Delineamento experimental para aumento da eficiência de provadores treinados. In: SIMPÓSIO IBERO-AMERICANO EM ANÁLISE SENSORIAL, 6., 2010, São Paulo. Anais do simpósio. São Paulo: Associação Brasileira de Ciências Sensoriais, 2010. 1 CD-ROM.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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14. | | FREITAS, D. de G. C.; CORREA, F. M.; SOUZA, A. L. S. e; ALCANTARA, M. de; GODINHO, J. C. C.; PEREIRA, P. C. de A.; DELIZA, R. Delineamento experimental para aumento da eficiência de provadores treinados. Brazilian Journal of Food Technology, Campinas, v. 14, p. 65-71, 2011. Edição especial.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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15. | | ARGÔLO FILHO, R. C.; COSTA, R. L.; PINHEIRO, H. E.; CORRÊA, F. M.; VALICENTE, F. H.; POMELLA, A. W. V.; LOGUERCIO, L. L. Requirement of simultaneous assessment of crystal- and supernatant-related entomotoxic activities of Bacillus thuringiensis strains for biocontrol-product development. Toxins, v. 6, p. 1598-1614, 2014.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
Biblioteca(s): Embrapa Milho e Sorgo. |
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16. | | GRADELA, A.; SOUZA, V. N.; QUEIROZ, M. M. de; CONSTATINO, A. da C.; BANDEIRA, C. G. C.; FARIA, M. D. de; MILANELO, L.; FRANZO, V. S.; CORRÊA, F. M. Biometria corporal e parâmetros hematológicos de Trachemys scripta elegans e Trachemys dorbignyi (Testudines: Emydidaae) criadas em cativeiro em Petrolina, Pernambuco. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 1, p. 83-90, janeiro. 2017.Biblioteca(s): Embrapa Unidades Centrais. |
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17. | | MIRANDA, J. P. L. de; SILVA, L. M. da; LIMA, R. L.; DONAGEMMA, G. K.; BERTOLINO, A. V. F. A.; FERNANDES, N. F.; CORREA, F. M.; POLIDORO, J. C.; TATO, G. K. Influência do pousio na recuperação de propriedades físicas de um Cambissolo em ambiente agrícola serrano - Bom Jardim (RJ). In: REUNIÃO BRASILEIRA DE MANEJO E CONSERVAÇÃO DO SOLO E DA ÁGUA, 17., 2008, Rio de Janeiro. Manejo e conservação do solo e da água no contexto das mudanças ambientais. Rio de Janeiro: Embrapa Solos, 2008. 1 CD-ROM. (Embrapa Solos. Documentos, 101).Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Solos. |
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18. | | BENEVENTI, M. A.; SHINOZAKI, K. Y.; NAKASHIMA, K.; NEPOMUCENO, A. L.; CORREA, F. M.; ROLLA, A. A. P. de SÁ MANDEL, S. M.; BEMQUERER, M. P.; OLIVEIRA, G. R.; NAKASU, E. Y. T.; ROCHA, T. L.; GROSSI de SÁ, M. F. Molecular analysis and biosafety of transgenic soybean drougth tolerance. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 2., 2009, Búzios. Programa e resumos. [S.l.]: SBG, 2009. p. 107.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Soja. |
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Registros recuperados : 18 | |
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Nenhum registro encontrado para a expressão de busca informada. |
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